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. 2017 Jun 19;21(12):3360–3371. doi: 10.1111/jcmm.13239

Figure 3.

Figure 3

PTTG3P promotes G1‐S cell cycle transition in GC cells. (A) EdU incorporation was evaluated using an EdU imaging kit. Quantitative analysis showed a greater number of EdU‐positive cells in the PTTG3P‐overexpression group compared to the control. Cells were counted by immunofluorescence microscopy under 200× magnification (scale bars = 100 μm). (B) Cell cycle distribution of AGS and HGC‐27 cells transfected with PTTG3P or a vector control was evaluated using a flow cytometer. (C) Western blotting analysis of the expression levels of cyclin D1 and p27. GAPDH was used as a reference. Data are shown as the mean ± SD of three replicates; **P < 0.01.