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. 2017 Jun 19;21(12):3360–3371. doi: 10.1111/jcmm.13239

Figure 4.

Figure 4

PTTG3P inhibits GC tumour cell apoptosis. (A) GC cells were transfected with PTTG3P or control vector and treated with or without 5‐FU for 24 hrs. Then, all the cells were stained with Annexin V and PI. The Annexin V−PI−, Annexin V+PI−, Annexin V−PI+ and Annexin V+PI+ subgroups indicate live cells, early apoptotic cells, necrotic cells and late apoptotic cells, respectively. (B) Western blotting analysis of the expression levels of PARP1, cleaved PARP1, caspase‐3 and cleaved caspase‐3. GAPDH was used as a reference. Data are shown as the mean ± SD of three replicates; *P < 0.05.