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. 2017 Aug 5;21(12):3730–3740. doi: 10.1111/jcmm.13282

Figure 5.

Figure 5

MiR‐139‐5p suppressed the proliferation of OSCC cells by targeting HOXA9. (A and B) Expression of miR‐139‐5p and HOXA9 mRNA was detected with qRT‐PCR in each group. (C) The protein level of HOXA9 in SAS and CAL‐27 cell lines was assessed by Western blot. GAPDH was used as an internal control. (D) Cell viability was analysed by MTT assay. (E) Cell proliferation was detected through colony formation assay. Data are expressed as mean ± S.D. **P < 0.01 compared with control in SAS cell line, ##P < 0.01 compared with control in CAL‐27 cell line. Control: control group, cells were without transfection. pEGFP‐NC: pEGFP‐NC group, cells transfected with empty pEGFP plasmids. pEGFP‐HOXA9: pEGFP‐HOXA9 group, cells transfected with pEGFP‐N1‐3FLAG‐HOXA9‐GFP plasmids. MiR‐139‐5p mimics + pEGFP‐HOXA9: miR‐139‐5p mimics + pEGFP‐HOXA9 group, cells cotransfected with miR‐139‐5p mimics and pEGFP‐HOXA9.