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. 2017 Jun 13;21(12):3277–3287. doi: 10.1111/jcmm.13231

Figure 5.

Figure 5

Effects on oxidative stress, endothelial nitric oxide synthase (eNOS) modulation and antioxidant system. (A) Using lucigenin‐enhanced chemiluminescence, superoxide anion production is expressed as the relative light unit (RLU) per second per gram of protein. (B) Substraction of lucigenin‐enhanced chemiluminescence with NG‐ nitro‐L‐arginine methyl ester (L‐NAME) incubation from total superoxide anion, presented as Δ L‐NAME. (C) Dihydroethidium (DHE) fluorescence quantification from LV sections. (D) Representative DHE‐stained sections. (E) Monomer:dimer ratio of eNOS. (F) Phosphorylation of eNOS at serine 1177. (G) S‐glutathionylation of eNOS. The representative co‐immunoprecipitation of eNOS without (top) and with (bottom) DTT to remove glutathionylation. Unspecific mouse IgG antibody was used as a negative control. (H) Ratio between oxidized glutathion (GSSG) and reduced glutathion (GSH). (I) Superoxide dismutase (SOD) activity. (J) Cardiac NO production analysed by total nitrate and nitrite. *P < 0.01 versus corresponding Sham; §P < 0.05 versus corresponding Sham; †P < 0.01 FA‐DOXO versus DOXO.