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. Author manuscript; available in PMC: 2019 Jan 28.
Published in final edited form as: Cancer Lett. 2017 Oct 26;413:82–93. doi: 10.1016/j.canlet.2017.10.029

Figure 6. Reduction of mutant p53 associates with increased mitochondrial apoptosis.

Figure 6

MIA PaCa-2 cells were plated. 24 h before NL exposure, cells were pretreated with 1, 5, or 10 µM Pifithrin-μ (PFT-μ) or Pifithrin-α (PFT-α) for p53 inhibition. Cells were then treated with 5 µM NL for 24 h. (A–B) Caspase-3, -8 and -9 activities in control and NL-treated MIA PaCa-2 cells previously treated with PFT-μ. (C) Caspase-3 activities in control and NL-treated MIA PaCa-2 cells previously treated with PFT-α. (D) Western Blot analysis of lysates from MIA PaCa-2 which were pretreated with 5 µM PFT-μ for 24 h, and then treated with 5 µM NL for 24 h. Data are mean ± SD, n = 3 and presented as fold-change compared to respective controls. Statistical significance was determined by a t-test: *p<0.05.