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. 2017 Jun 29;21(12):3467–3480. doi: 10.1111/jcmm.13256

Figure 3.

Figure 3

The angiogenic capacity of RF/6A was inhibited when treated with conditioned media from hypoxic BV2 being transfected with basigin‐2 siRNA (BSG2 siRNA). (A) Messenger RNA of BSG2 and Protein expression of BSG in BV2 cells transfected with either BSG2 siRNA or Mock siRNA. **P < 0.01 versus nontreated group; # P < 0.05 versus both siRNA sequence used group; ## P < 0.01 versus both siRNA sequence used group. (B) Transwell migration assay examining the directional migration of RF/6A cells. The cell migration assay was performed as described in the Materials and Methods section. Scale bar = 500 μm. **P < 0.01 versus normal group; ## P < 0.01 versus hypoxia BSG2 siRNA group; NS no significant difference during groups. (C) Representative images and quantification of in vitro tube formation analysis in Matrigel. The formation of capillary‐like structures was photographed at 6, and 20 hrs. Scale bar = 500 μm. **P < versus RF/6A group; ## P < 0.01 versus CM Mock siRNA group.