Figure 7.

JAM‐A protein expression is diminished in the colon of CerS2 null mice. Mice were exposed to 2% (w/v) DSS in the drinking water for 7 days to induce colitis or normal plain water (control). (A) Representative Western blots of apical junctional complex proteins (upper panels) and quantification (lower panels) (n = 5) of JAM‐A and occludin in colon. *P < 0.05. (B) JAM‐A expression (green) in the colon upon DSS treatment in WT and CerS2 null mice was examined using immunofluorescence; nuclei are counterstained with DAPI (blue) (200× magnification). ZO‐1, zonula occludens‐1.