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. 2017 Jun 23;21(12):3394–3404. doi: 10.1111/jcmm.13250

Figure 1.

Figure 1

Doxorubicin induces apoptosis in HL‐1 cardiac myocytes. A and B show caspase‐3 cleavage by immunoblot and densitometry; C and D show PARP1 cleavage by immunoblot and densitometry. Tubulin and β‐actin served as a loading control. (A–D) Cells were stimulated with 1 μmol/l DOX and then harvested at the indicated time for immunoblotting. Figures presented are the representative figures of at least three independent experiments. The densitometry data were expressed as the mean ± SEM of three independent experiments. E shows DNA fragmentation. Cells were stimulated with 1 μmol/l DOX at indicated time‐points and apoptosis‐related DNA fragmentation were analysed using the cell death detection ELISA (E). Data were expressed as the mean ± SEM of three independent experiments. ns, non‐significant, *P < 0.05, ***P < 0.001, ****P < 0.0001 versus 0 hr.