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. 2017 Jun 23;21(12):3394–3404. doi: 10.1111/jcmm.13250

Figure 2.

Figure 2

Doxorubicin‐induced mitochondrial fission is associated with up‐regulation in Mtfp1 expression. (A and B) doxorubicin (DOX) induces mitochondrial fission in HL‐1 cells. Cells were stimulated with 1 μmol/l DOX at indicated time‐points and mitochondrial morphology was analysed. A shows mitochondrial morphology. B shows percentage of cells undergoing mitochondrial fission. Data were expressed as the mean ± SEM of three independent experiments. (C and D) DOX up‐regulates mitochondrial fission process 1 (Mtfp1) expression in mitochondria in a dose‐ and time‐dependent manner. Analysis of Mtfp1 expression. HL‐1 cells were stimulated with the indicated doses of DOX and harvested at 6 hrs (C, upper panel), and cells were stimulated with 1 μmol/l DOX and then harvested at the indicated time (D, upper panel) for immunoblotting. Cytochrome‐c oxidase (Cox4) served as a loading control for mitochondrial fraction. Figures presented are the representative figures of at least three independent experiments. The densitometry data were expressed as the mean ± SEM of three independent experiments (C and D lower panels). *P < 0.05, **P < 0.01, ****P < 0.0001 versus non‐treatment.