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. 2017 Jun 23;21(12):3394–3404. doi: 10.1111/jcmm.13250

Figure 3.

Figure 3

Knockdown of Mtfp1 can prevent HL‐1 cell from doxorubicin‐induced mitochondrial fission and apoptosis. (A) Analysis of mitochondrial fission process 1 (Mtfp1) expression. Immunoblot shows Mtfp1 knockdown in HL‐1 cells. β‐actin served as a loading control. The densitometry data were expressed as the mean ± SEM of three independent experiments (lower panel). B shows mitochondrial morphology. Bar = 2 μm. C shows percentage of cells with mitochondrial fission. Cells were exposed to a higher concentration (2 μmol/l) doxorubicin (DOX) and mitochondrial fission was analysed by MitoTraker Red (B and C). D shows TUNEL‐positive cells. Bar = 20 μm. E shows percentage of TUNEL‐positive cells. F shows apoptosis‐related DNA fragmentation. Cells were exposed to a higher concentration (2 μmol/l) DOX, and apoptosis was analysed by TUNEL assay (D and E), and DNA fragments were analysed using the cell death detection ELISA (F). Figures presented are the representative figures of at least three independent experiments. Data were expressed as the mean ± SEM of three independent experiments. ***P < 0.001 and ****P < 0.0001.