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. 2017 Jun 23;21(12):3394–3404. doi: 10.1111/jcmm.13250

Figure 4.

Figure 4

Enforced expression of Mtfp1 sensitizes HL‐1 cells to doxorubicin‐induced mitochondrial fission and apoptosis. (A) Analysis of Mtfp1 expression. Immunoblot shows Mtfp1 overexpression in HL‐1 cells (upper panel). β‐actin served as a loading control. The densitometry data were expressed as the mean ± SEM of three independent experiments (lower panel). (B) Enforced expression of Mtfp1 sensitizes cells to undergo DOX‐induced mitochondrial fission. HL‐1 cells were exposed to a lower concentration of DOX. B shows percentage of cells with mitochondrial fission; (C and D) Enforced expression of Mtfp1 sensitizes cells to undergo DOX‐induced apoptosis. Cells were exposed to a lower concentration (0.3 μmol/l), and percentages of apoptosis were analysed by TUNEL assay (C) and DNA fragments were analysed using the cell death detection ELISA (D). Data were expressed as the mean ± SEM of three independent experiments. Figures presented are the representative figures of at least three independent experiments. ***P < 0.001 and ****P < 0.0001.