Figure 1.

Knockdown of AEG‐1 expression suppressed EMT, migration and invasion of gastric cancer cells. A, SGC7901 and MGC803 cells with stable AEG‐1 knockdown (AEG‐1_shRNA) or control cells (scramble_shRNA) were cultured for 24 hrs and then subjected to Western blot assay. B and C, transwell migration assay (B) and Matrigel invasion assay (C) of AEG‐1 knockdown cells. Cell lines were seeded into the upper chamber in serum‐free medium. The lower chamber was filled with conditioned medium containing 10% FBS. After 24 hrs, cells in the lower chamber were subjected to the assay and observed under microscopy. The average number of cells in the lower chamber was quantified. Magnification, 100×. Columns, means of five fields; bars, S.D. *P < 0.05. The data are representatives of three independent experiments.