Figure 6.

Silencing of AEG‐1 expression suppressed tumour growth and metastasis in xenograft and orthotopic nude mouse models, respectively, in parallel with downregulation of eIF4E, MMP‐9 and Twist protein levels. A, B and C, tumour size (A), tumour weight (B) and tumour images (C) of initial AEG‐1 knockdown (AEG‐1_shRNA) and control tumours (scramble_shRNA) evaluated in the xenograft nude mouse model. Points, means of six tumours; bars, S.D. (A). Horizontal lines, means of six tumours; points, tumour weight of each mouse (B). D, cell lysates of each tumour were prepared and subjected to Western blot assay. E, the number of metastases from AEG‐1 knockdown tumours and control tumours were quantified in the orthotopic nude mouse model. Horizontal lines, means of 10 mice; points, metastatic tumour number in each mouse. *P < 0.05.