Figure 1.
Overexpression of NmU correlates with increased TGFβ1 and PD-L1 levels. (A), (B), and (C), TGFβ1 levels were determined by ELISA in serum-free conditioned medium of HCC1954 and SKBR3 cell variants and expressed as pg TGFβ1 per mg total cell protein. (D) and F, HCC1954 cell variants were stained with APC-labeled anti-PD-L1, followed by FACS analysis in a FACSCanto II flow cytometer. (E) and (G), Same as in (D) for SKBR3 cell variants. Results represent averaged replicates from at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.