Table 1.

Chemical Composition of Synthesized Oligonucleotides

		2′ Ribose nucleotide modifications
Identifier	5′ Modifications	A	C	G	T/U	3′ Nucleotide
DNA T	S	H	H	H	H	Inverted dT
DNA C	S	H	H	H	H	2′-Deoxy C
fYrR T	S	OH	F	OH	F	Inverted dT
fYrR C	S	OH	F	OH	F	2′-Fluoro C
fGmH T	S	OMe	OMe	F	OMe	Inverted dT
fGmH C	S	OMe	OMe	F	OMe	2′-O-methyl C
OMe T	S	OMe	OMe	OMe	OMe	Inverted dT
OMe C	S	OMe	OMe	OMe	OMe	2′-O-methyl C
A9.min	S	OH	F	OH	F	dT

We synthesized A9.min, an anti-PSMA aptamer, as well as eight variants of C36, a nontargeting control sequence. Their 5′ and 3′ end and 2′ ribose modifications are as indicated. S indicates a 5′ thiol modifier C6 modification. Note that C36 oligonucleotides synthesized with an inverted dT column contain 37 nucleotides while those synthesized on a C column contain 36 nucleotides. fYrR C was synthesized on a universal support column because of the lack of availability of 2′-fluoro C columns. After cleavage from the column, the first 3′ nucleotide is 2′-fluoro C.

PSMA, prostate specific membrane antigen.