Skip to main content
. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: Chem Commun (Camb). 2017 Nov 28;53(95):12778–12781. doi: 10.1039/c7cc07732g

Fig. 3.

Fig. 3

Antibacterial activity of NicA and other isolated molecules. UPEC was cultured for 18 h in (a) MOPS-Fe or (b) MOPS-Fe supplemented with 40 μM FeSO4 and the indicated pure molecule (x-axis). Growth was quantified by measuring OD600. (c) Iron chelation was quantified using the chrome azural S (CAS) assay and a decrease in absorbance at 630 nm represents iron chelation. The shading of the gray bars indicates the final concentration of the pure molecules. Shown is an average of 3 replicates. Error bars represent one standard deviation from the mean. Significance was calculated using an unpaired t test in Prism and determined using the Holm-Sidak method with alpha = 0.05. Each row was analyzed individually without assuming a consistent standard deviation where *, p < 0.05; **, p < 0.01; ***, p < 0.001; #, p < 0.0001.