Fig 2.

(a) Gene expression correlations between single cells from replicate experiments. The Spearman correlations observed are similar across replicates, and are just slightly lower than bulk controls (0.67 vs 0.75). In contrast, when these single-cell H1650 transcriptomes are compared to those of white blood cells, very low correlation is observed. Color of axis labels illustrate specific sample types, with red representing bulk white blood cell samples, black representing bulk H1650 cell samples, and purple, green and blue representing single H1650 cells from 3 separate replicate experiments. (b) Sample scatter plots illustrating correlation in gene expression between white blood cells (WBCs) and H1650 cells. The single-cell data (3 randomly chosen examples shown) match the H1650 bulk sample (50 cells bulk), while having little correlation with the bulk WBC sample (10 WBC bulk).