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. 2017 Nov 29;12(11):e0188748. doi: 10.1371/journal.pone.0188748

Fig 3. Cellular expression of LC3 and Beclin1 following ischemic stroke.

Fig 3

A, Double-staining for LC3 (red) and NeuN (green) showed that numerous LC3-positive cells were colocalized within neurons in the cortex (A1-A3). However, staining against LC3 and GFAP showed that very few LC3-positive cells were colocalized with astrocytes in the lesion core surrounded areas, 1 day post stroke (A4-A6). B, Immunofluorescent staining against Beclin1 (red) and GFAP (green) showed that Beclin1 positive cells were mainly colocalized with neurons in the cortex instead of astrocytes (B1-B3). Scale bar represents 20 μm. C, The expression of LC3 and Beclin1 was assessed by western blotting at 6 h, 1 day, 3 days, and 7 days post injury. D, The expression of autophagosomes peaked at 3 days and then decreased slightly at 7 days. (n = 4 for each group, *p < 0.05 vs. sham group, ** p < 0.01 vs. sham group, one-way ANOVA with Tukey post hoc test).