Fig 4. Condensation is irreversible in scc2-4 mutants.

A) Flow cytometer data reveals DNA content throughout the experimental analyses. Cells were maintained in nocodazole, post-alpha factor release, for 3 hours at 37°C followed by an additional 1 hour at 23°C. B) Chromosome mass and rDNA structures detected using DAPI in wildtype and scc2-4 mutant strains. C) Quantification of condensed (loop/line) and decondensed (puff/other) rDNA populations in wildtype (YBS1019) and scc2-4 mutant (YMM551) cells. Quantifications and statistical analyses of rDNA condensation were obtained from 3 biological replicates for each strain (wildtype and scc2-4 mutant cells) in which each replicate included 100 cells for each strain. Statistical analyses of condensed populations were performed using one-way ANOVA followed by post-hoc Tukey HSD Test (p = 0.001 for wildtype versus scc2-4 mutant cell rDNA condensation at 37°C; p = 0.001 for wildtype versus scc2-4 mutant cell rDNA condensation at 23°C; p = 0.890 for wildtype cell rDNA condensation at 37°C versus 23°C; p = 0.301 for scc2-4 mutant cell rDNA condensation at 37°C versus 23°C). Statistical significant differences (*) are based on p < 0.05.