Fig 9. LOPAC hits inhibit IP6K2 and IP6K3.
Dose response assay for inhibition of (A) IP6K2 and (B) IP6K3 by Myricetin and 6 -Hydroxy-DL-Dopa. Myricetin and 6-Hydroxy-DL-Dopa were able to inhibit IP6K2 with an IC50 of 23.41±1.10μM and 1.66±1.06μM respectively and inhibit IP6K3 with an IC50 of 4.93±1.14μM and 11.10±1.13μM respectively. Reactions were run with (A) 1mM ATP, 100μM IP6, 7.5nM IP6K2, for 30 minutes at 37°C or (B) 1mM ATP, 100μM IP6, 120nM IP6K3, for 120 minutes at 37°C. Percent activity is defined as 100*(μexperimental-μnegative)/(μpositive–μnegative) where positive is the experimental result with no inhibitor present and negative is the signal produced at the highest concentration of inhibitor.