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. 2017 Nov 29;12(11):e0188885. doi: 10.1371/journal.pone.0188885

Fig 2. Effects of PEF on the EGCG-induced growth inhibition in PANC-1 and HEK293 cells.

Fig 2

The cell viability (24 h) and the cell number (72 h) were determined using MTT assay. PANC-1 cells were treated with various concentrations of EGCG alone or combined with consecutive PEF (60 V/cm in amplitude, 2 ms in duration, and 2 Hz in frequency) for (A) 24 h or (B) 72 h. EGCG (0–50 μM) alone concentration-dependently inhibited PANC-1 cells, whereas co-treatment with EGCG and the PEF together enhanced the inhibition. (C) PANC-1 cells were treated with EGCG (0–20 μM) alone or combined with various strengths of PEF for 24 h. (D) HEK293 cells were treated EGCG (0–30 μM) alone or combined with PEF (60 or 90 V/cm) for 24 h.