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. 2017 Nov 29;12(11):e0188885. doi: 10.1371/journal.pone.0188885

Fig 6. Effects of the combination treatment on the inhibition of PANC-1 migration.

Fig 6

Effects of combination treatment on the migratory potential of PANC-1 cells were analyzed using wound healing assay. After scratch gap was created, PANC-1 cells were treated for 24 h with EGCG (10 μM or 20 μM) alone or combined with 60 V/cm PEF. (A) Representative pictures showed the scratch (wound) at t  =  0 h and 24 h. (B) The areas of cell-free gaps were measured and quantified using ImageJ. Wound closure rate was determined as the percentage of area closed after 24 h from the initial wound area. These results revealed combination treatment with EGCG and PEF significantly reduced the migration ability of PANC-1 cells. The “+” means that the PEF was involved in the treatment, and the “-” means the PEF was not involved. (***, p<0.001 compared to corresponding control).