Table 11. Stratified analyses between PADI4 polymorphism rs2477137 and ESCC risk by sex, age, smoking status and alcohol consumption.
| Variable | rs2477137 C>A (case/control) a | Adjusted OR b (95%CI); p; phc | |||||||
|---|---|---|---|---|---|---|---|---|---|
| CC | AC | AA | AC+AA | CC | AC | AA | AC+AA | AA vs. (CC+AC) | |
| Sex | |||||||||
| Male | 290/300 | 137/137 | 10/16 | 147/153 | 1.00 | 0.97(0.73-1.28); p:0.827; ph:1.000 |
1.55(0.69-3.46); p:0.321; ph:0.805 |
1.00(0.76-1.33); p:1.000; ph:0.999 |
1.56(0.70-3.48); p:0.322; ph:0.156 |
| Female | 109/147 | 65/65 | 8/11 | 73/76 | 1.00 | 0.74(0.48-1.13); p:0.194; ph: 1.000 |
1.02(0.39-2.62); p:1.000; ph:0.805 |
0.77(0.52-1.16); p:0.216; ph:0.999 |
1.13(0.44-2.87); p:1.000; ph:0.156 |
| Age | |||||||||
| <63 | 210/241 | 86/104 | 5/12 | 91/116 | 1.00 | 1.05(0.75-1.48); p:0.795; ph:0.073 |
2.09(0.73-6.03); p:0.217; ph:0.262 |
1.11(0.79-1.55); p:0.556; ph:0.049 |
0.49(0.17-1.39); p:0.220; ph:0.167 |
| ≥63 | 189/206 | 116/98 | 13/15 | 129/113 | 1.00 | 0.78(0.56-1.08); p:0.149; ph:0.073 |
1.06(0.49-2.28); p:1.000; ph:0.262 |
0.80(0.58-1.11); p:0.192; ph:0.049 |
0.86(0.40-1.85); p:0.847; ph:0.167 |
| Smoking status | |||||||||
| Never | 216/317 | 119/151 | 12/22 | 131/173 | 1.00 | 0.87(0.64-1.16); p:0.363; ph:0.001 |
1.25(0.61-2.58); p:0.593; ph:0.263 |
0.90(0.68-1.19); p:0.512; ph:0.000 |
0.76(0.37-1.56); p:0.484; ph:0.000 |
| Ever | 183/130 | 83/51 | 6/5 | 89/56 | 1.00 | 0.86(0.57-1.31); p:0.529; ph:0.001 |
1.17(0.35-3.93); p:1.000; ph:0.263 |
0.89(0.59-1.33); p:0.61; ph:0.000 |
0.82(0.25-2.72); p:0.746; ph:0.000 |
| Alcohol consumption | |||||||||
| Never | 264/337 | 138/156 | 16/24 | 154/180 | 1.00 | 0.88(0.67-1.17); p:0.431; ph:0.045 |
1.18(0.61-2.26); p:0.742; ph:1.000 |
0.92(0.70-1.19); p:0.537; ph:0.038 |
0.82(0.43-1.56); p:0.627; ph:0.000 |
| Ever | 135/110 | 64/46 | 2/3 | 66/49 | 1.00 | 0.88(0.56-1.39); p:0.64; ph:0.045 |
1.84(0.30-11.21); p:0.661; ph:1.000 |
0.91(0.58-1.43); p:0.733; ph:0.038 |
0.52(0.09-3.17); p:0.658; ph:0.000 |
a The genotyping success rate was 98.47% for rs2477137 C>A; b Adjusted for age, sex, smoking status and alcohol consumption (besides stratified factors accordingly) in a logistic regression model; c ph for heterogeneity; Bold values are statistically significant (p<0.05).
PADI4 rs2477137 C>A polymorphism was not associated with the ESCC susceptibility. Elder cohort (≥ 63 years) had increased susceptibility to ESCC in the dominant model (ph=0.049). In rs2477137 AC genotype, smoking increased susceptibility to ESCC (ph=0.001). Smoking was associated with ESCC risk in the dominant (ph=0.000) and recessive (ph=0.000) models. In rs2477137 AC genotype, alcohol drinking increased susceptibility to ESCC (ph=0.045). Alcohol drinking was associated with ESCC risk in the dominant (ph=0.038) and recessive (ph=0.000) models.