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. 2017 Oct 3;8(55):93688–93703. doi: 10.18632/oncotarget.21458

Figure 3. Caspase-8-dependent inhibition of tumor growth.

Figure 3

(A) p95HER2/611CTF cells stably expressing a Scrambled or a Caspase 8-targeting shRNA were cultured either in complete or starvation medium and apoptosis was measured (left panel). Caspase-8 knockdown was determined by western blotting (right panel). Error bars, SD from three independent experiments. ***P < 0.001. (B) Subcutaneous growth of p95HER2/611CTF cells stably expressing a Scrambled or a Caspase 8-targeting shRNA injected in SCID-bBeige mice. Representative pictures were taken at day 30 after injection. (C) End-stage tumor weight in mice injected with p95HER2/611CTF cells stably expressing either a Scrambled oligonucleotide or a Caspase-8-targeting shRNA (n = 8). Graphs shown mean ± SEM. *P < 0.05. (D) Representative images and quantification of CD31+ tumor vessel area in mice injected either with p95HER2shScr or p95HER2shCasp8 cells (n = 8). The vessel area was calculated by the percentage of CD31 area per field.