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. 2017 Oct 3;8(55):93688–93703. doi: 10.18632/oncotarget.21458

Figure 6. Role of mTOR complexes in apoptosis induced by metabolic stress.

Figure 6

(A) Cells were cultured in Stv medium for the indicated times. Following these treatments, mTORC1 and mTORC2 activation (p-p70/S6K, p-4EBP1 and p-AktSer473), was assessed by western blotting. Results are representative of 4 independent experiments. (B) Left panel shows apoptosis in p95HER2/611CTF cells cultured in complete or stv medium in the presence or absence of Torin-1 (250 nM). FLIP levels and mTORC1/mTORC2 activities were determined by western blot analysis (right panels). (C) Apoptosis in p95HER2/611CTF cells stably expressing a Scrambled, Raptor or Rictor shRNA cultured either in complete or stv medium (left panel). Where indicated, Rapamycin (5 µM) or Torin (250 nM) were added to the medium. Levels of FLIP, Raptor and Rictor proteins upon 16 h of treatment are shown in the right panel. Error bars, SD from three independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05. (D) Schematic overview of the differential response to metabolic stress in control and p95HER2/611CTF cells.