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. 2017 Sep 8;8(55):93757–93770. doi: 10.18632/oncotarget.20771

Figure 2. Knockdown of LC3 inhibited the expression of collagen matrix and affects the architecture arrangement of α-SMA in HSFs.

Figure 2

HSFs, with 50-70% confluent, were infected by AdLC3, shLC3 and their negative control adenvirus vectors, cultured in DMEM medium for 36-48 h, and then protein expression levels were detected by Western blot. (A, B) α-SMA protein expresses and changes in the α-SMA/β-actin ratio. (A, C) Col 1 protein expresses and changes in the Col 1/β-actin ratio. (A, D) Col 3 protein expresses and changes in the Col 3/β-actin ratio. Data expressed as the mean ± SEM; n = 3, ▲p > 0.05, *p < 0.05, **p < 0.01 compared with the negative control. (E) The architecture arrangement of α-SMA in infected HSFs (with GFP protein) was analyzed using a specific mAb and a Cy3-conjugated secondary antibody. Fibroblast nuclei were stained with DAPI. Scale bars, 50 μm.