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. 2017 Sep 27;8(55):93924–93941. doi: 10.18632/oncotarget.21370

Figure 7. Klf4 suppressed EMT-like cellular marker alterations and decreased migration and invasion of NPC cells through inducing E-cadherin expression.

Figure 7

(A) ChIP assay was performed using anti–Klf4 antibody or IgG antibody to identify Klf4 binding sites on the E-cadherin promoter in HONE1 and 5-8F cells. (B) Cell extracts from HONE1 and 5-8F cells infected with LV-con, LV-Klf4 or LV-Klf4 plus shE-cad (E-cad: E-cadherin) were analyzed by immunoblotting with antibodies against the indicated proteins. (C-D) Klf4 inhibited the motility (C) and invasion (D) of HONE1 and 5-8F cells through inducing E-cadherin expression. The motile and invasive activities of the indicated NPC cells were analyzed using transwell (C) and Boyden (D) assays, respectively. Original magnification: 200×.