Figure 1. IL-10-producing Breg cells exhibit lower miR-21 expression than non-Breg cells.
(A) CD4+ T cells and CD19+ B cells were purified by MACS from the spleens of EAE mice at disease onset (day 14), and miR-21 expression was analyzed by qRT-PCR using U6 snRNA as an endogenous control. (B, C) CD5+CD1dhi and CD5-CD1d- B cells (B) and TIM-1+ and TIM-1- B cells (C) were sort-purified from the spleens of EAE mice at the onset stage (day 14), and miR-21 expression was analyzed by qRT-PCR using U6 snRNA as an endogenous control. The data are representative of 3 independent experiments. The bars and error bars represent the mean ± SEM. **P < 0.01.