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. 2017 Oct 6;8(55):94129–94141. doi: 10.18632/oncotarget.21588

Figure 4. A P53 inhibitor blocks apoptosis and promotes necroptosis in A549 cells.

Figure 4

(A) Phase-contrast-fluoresence microscopy of cells in the FLAG group (a-c), FLAG-B2 group (d-f), FLAG + Pif α group (g-i) and FLAG-B2 + Pif α (30 μM) group (j-l) stained with Annexin-V-FITF (b, e, h, and k) and PI (c, f, i, and l) at 48 h post-transformation. (B) Quantification of fluorescence of Annexin-V and PI. Error bars represent the SEM of 3 independent experiments. All data were analyzed using a paired or unpaired Student’s t-test, as appropriate. *P < 0.05 indicates statistical significance. (C) Influence of a P53 inhibitor on expression of apopotic and necrotic genes in A549 cells at 48 h post-transfection (western blotting). (D) Quantitation of western blotting results. Error bars represent the SEM of 3 independent experiments. All data were analyzed using a paired or unpaired Student’s t-test, as appropriate. *P < 0.01 indicates statistical significance.