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. 2017 Oct 7;8(55):94166–94171. doi: 10.18632/oncotarget.21607

Figure 2. Genome editing efficiency comparison showed pgRNA-BL is the most efficient form of gRNA.

Figure 2

(A) Percentage of GFP positive cells via GFP reporter assay when cells transfected with different forms of gRNA plus Cas9. N=3. * indicates P<0.05. (B) The position of restriction enzyme sites and gRNA sites for hDES1 and hDES2. (C, D) NHEJ efficiency was measured by restriction enzyme site destruction assay for two sites on human gene Desmin. Up-panel showed representative figures of restriction enzyme digestion and gel electrophoresis; down-panel showed un-digested band density measured by Image J (n=3). * indicates P<0.05. NC: negative control; JKJ: pgRNA-JKJ; BDR: pgRNA-BDR; BSH: pgRNA-BSH; BL: pgRNA-BL; CL: pgRNA-CL; hDES1-3: three target sites on human gene Desmin.