Heavy chain variable region forward primer |
VHF1 |
GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTGGTGCAGTCTGG |
VHF2 |
GCTGCCCAACCAGCCATGGCCCAGATCACCTTGAAGGAGTCTGG |
VHF3 |
GCTGCCCAACCAGCCATGGCCGAGGTGCAGCTGGTGSAGTCTGG |
VHF4 |
GCTGCCCAACCAGCCATGGCCGAGGTGCAGCTGKTGGAGTCTG |
VHF5 |
GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTGCAGGAGTCGGG |
VHF6 |
GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTACAGCAGTGGGG |
Heavy chain variable region reverse primer |
VHR1 |
CGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC |
VHR2 |
CGATGGGCCCTTGGTGGAGGCWGRGGAGACGGTGACCAGGGTBCC |
Light chain variable region forward primer |
VLF1 |
GGGCCCAGGCGGCCGAGCTCCAGATGACCCAGTCTCC |
VLF2 |
GGGCCCAGGCGGCCGAGCTCGTGATGACYCAGTCTCC |
VLF3 |
GGGCCCAGGCGGCCGAGCTCGTGWTGACRCAGTCTCC |
VLF4 |
GGGCCCAGGCGGCCGAGCTCACACTCACGCAGTCTCC |
Light chain variable region reverse primer |
VLR1 |
GAAGACAGATGGTGCAGCCACAGT |
Heavy chain variable region primer |
HF1 |
CATATGCAGGTGCAGCTGGTGCAGTCTG |
HR1 |
TGGGCCCTTGGTGGAGGCGGAGACGGTGACCAGGGTTC |
Light chain variable region primer |
LF1 |
CCATGGGCGAGCTCGTGATGACCCAG |
LR1 |
CAGCCTTGGGCTGACCTTTTATTTCCAACTTTGTC |
Constant region CH1 primer |
HF2 |
GAACCCTGGTCACCGTCTCCGCCTCCACCAAGGGCCCA |
HR2 |
GGTACCTTAAGAAGCGTAGTCCGGAAC |
Constant region CL primer |
LF2 |
GACAAAGTTGGAAATAAAAGGTCAGCCCAAGGCTG |
LR2 |
AAGCTTTTATGAACATTCTGTAGGGGCCACT |
Heavy chain primer |
HF1 |
CATATGCAGGTGCAGCTGGTGCAGTCTG |
HR2 |
GGTACCTTAAGAAGCGTAGTCCGGAAC |
Light chain primer |
LF1 |
CCATGGGCGAGCTCGTGATGACCCAG |
LR2 |
AAGCTTTTATGAACATTCTGTAGGGGCCACT |