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. 2017 Oct 7;8(55):94210–94222. doi: 10.18632/oncotarget.21618

Table 1. Primers used for the construction of the ROR1-cFab gene.

Primer name DNA sequence
Heavy chain variable region forward primer
VHF1 GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTGGTGCAGTCTGG
VHF2 GCTGCCCAACCAGCCATGGCCCAGATCACCTTGAAGGAGTCTGG
VHF3 GCTGCCCAACCAGCCATGGCCGAGGTGCAGCTGGTGSAGTCTGG
VHF4 GCTGCCCAACCAGCCATGGCCGAGGTGCAGCTGKTGGAGTCTG
VHF5 GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTGCAGGAGTCGGG
VHF6 GCTGCCCAACCAGCCATGGCCCAGGTGCAGCTACAGCAGTGGGG
Heavy chain variable region reverse primer
VHR1 CGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC
VHR2 CGATGGGCCCTTGGTGGAGGCWGRGGAGACGGTGACCAGGGTBCC
Light chain variable region forward primer
VLF1 GGGCCCAGGCGGCCGAGCTCCAGATGACCCAGTCTCC
VLF2 GGGCCCAGGCGGCCGAGCTCGTGATGACYCAGTCTCC
VLF3 GGGCCCAGGCGGCCGAGCTCGTGWTGACRCAGTCTCC
VLF4 GGGCCCAGGCGGCCGAGCTCACACTCACGCAGTCTCC
Light chain variable region reverse primer
VLR1 GAAGACAGATGGTGCAGCCACAGT
Heavy chain variable region primer
HF1 CATATGCAGGTGCAGCTGGTGCAGTCTG
HR1 TGGGCCCTTGGTGGAGGCGGAGACGGTGACCAGGGTTC
Light chain variable region primer
LF1 CCATGGGCGAGCTCGTGATGACCCAG
LR1 CAGCCTTGGGCTGACCTTTTATTTCCAACTTTGTC
Constant region CH1 primer
HF2 GAACCCTGGTCACCGTCTCCGCCTCCACCAAGGGCCCA
HR2 GGTACCTTAAGAAGCGTAGTCCGGAAC
Constant region CL primer
LF2 GACAAAGTTGGAAATAAAAGGTCAGCCCAAGGCTG
LR2 AAGCTTTTATGAACATTCTGTAGGGGCCACT
Heavy chain primer
HF1 CATATGCAGGTGCAGCTGGTGCAGTCTG
HR2 GGTACCTTAAGAAGCGTAGTCCGGAAC
Light chain primer
LF1 CCATGGGCGAGCTCGTGATGACCCAG
LR2 AAGCTTTTATGAACATTCTGTAGGGGCCACT