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. 2017 Oct 9;8(55):94235–94246. doi: 10.18632/oncotarget.21670

Figure 3. HDAC5 represses MKL1 mediated pro-inflammatory transcription.

Figure 3

(A) Different promoter constructs were transfected into HEK293 cells with MKL1 and/or HDAC5 expression construct. Luciferase activities were normalize by protein concentration and GFP fluorescence. (B) Different promoter constructs were transfected into HEK293 cells with HDAC5 expression construct followed by treatment with TNF-α (10ng/ml) for 12 hours. Luciferase activities were normalize by protein concentration and GFP fluorescence. (C) Different promoter constructs were transfected into RAW264 cells with MKL1 and/or HDAC5 shRNA construct. Luciferase activities were normalize by protein concentration and GFP fluorescence. (D) Different promoter constructs were transfected into RAW264 cells with HDAC5 shRNA construct followed by treatment with TNF-α (10ng/ml) for 12 hours. Luciferase activities were normalize by protein concentration and GFP fluorescence. (E) THP-1 cells were transfected with siRNA targeting HDAC5 or scrambled siRNA (SCR) followed by treatment with TNF-α (10ng/ml) for 12 hours. Expression of pro-inflammatory mediators was measured by qPCR. All experiments have been repeated three times.