Figure 6. HDAC5 disrupts the interaction between MKL1 and p65.

(A) FLAG-tagged MKL1 and V5-tagged p65 were transfected into HEK293 cells in the presence or absence of HDAC5. Immunoprecipitation was performed with anti-FLAG. (B) THP-1 cells were transfected with siRNA targeting HDAC5 or scrambled siRNA (SCR) followed by treatment with TNF-α (10ng/ml). Immunoprecipitation was performed with anti-MKL1. (C) THP-1 cells were transfected with HDAC5 expression construct or an empty vector (EV) followed by treatment with TNF-α (10ng/ml). Re-ChIP assays were performed with indicated antibodies. (D) THP-1 cells were transfected with siRNA targeting HDAC5 or scrambled siRNA (SCR) followed by treatment with TNF-α (10ng/ml). Re-ChIP assays were performed with indicated antibodies. (E) A schematic model. All experiments have been repeated three times.