Figure 1. The inhibitory effect of nucleosides on proliferation of Bel-7402 and Hela cells.

Four nucleosides adenosine, guanosine, thymidine, inosine and adenine were tested for inhibitory effect on cancer cell lines Bel-7402 and Hela. Adenosine was used at the final concentration of 1mg/ml (3.74mM), and equal molar of the other nucleosides guanosine, thymidine, inosine and adenine (1.06mg/ml, 0.91mg/ml, 1mg/ml and 0.52mg/ml respectively) were used. The cells were treated for 48h and 72h. The inhibitory effect on cancer cell growth was evaluated by MTT assay. The experiment was repeated for three times. Statistically significant differences compared with adenine and the other nucleosides are shown. ^*P < 0.05, ^**P < 0.01.