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. 2017 Oct 10;8(55):94462–94480. doi: 10.18632/oncotarget.21776

Figure 3. Growth analysis of the BoHV-1 recombinant viruses.

Figure 3

(A) Growth of the BoHV-1, vBoHV-1-∆UL21, vBoHV-1-∆UL21R and vBoHV-1UL21-HA viruses. For the single-step replication analysis, MDBK cells were infected with the indicated viruses at a MOI of 3, and harvested at 6, 12, 18, 24, 30, and 48 hpi. A t-test of the data revealed very significant differences between the BoHV-1 and vBoHV-1-∆UL21 viruses in terms of their extracellular (**, p < 0.01) and intracellular (**, p < 0.01) viral titers, while no significant (ns) difference was observed between the BoHV-1, vBoHV-1-∆UL21R and vBoHV-1UL21-HA viruses (p > 0.05). (B) Plaque size and morphology of the BoHV-1, vBoHV-1-∆UL21, vBoHV1-∆UL21R and vBoHV-1UL21-HA viruses. MDBK cells were infected with serial dilutions of each virus in 24-well plates and overlaid with methylcellulose. At 48 hpi, plaques were fixed with formaldehyde and stained with crystal violet. (C) The mean plaque sizes of the aforementioned viruses were determined with an Olympus IX70® Microscope. The difference in the mean plaque size of the vBoHV-1-∆UL21 virus was shown to be significantly smaller using a t-test (***, p < 0.001). Each independent experiment was performed three times.