Skip to main content
. 2017 Oct 10;8(55):94462–94480. doi: 10.18632/oncotarget.21776

Figure 4. Localization of UL21 using confocal microscopy.

Figure 4

(A) MDBK cells were infected with the vBoHV-1-UL21-HA virus at a MOI of 3 for 18 hrs. The infected cells were incubated with primary antibodies (mouse anti-HA) and rabbit polyclonal antisera, and then stained with Cy3-conjugated goat anti-mouse and fluorescein isothiocyanate-conjugated goat anti-rabbit IgG antibodies, Nuclei were stained blue with DAPI. Then, mount with anti-fade mounting medium. Specific capsid fluorescence was visualized under a Zeiss LSM 880 laser-scanning confocal microscope (B) mock-infected cells.