Figure 8. Quantitation of the virion distribution within cells using TEM.
MDBK cells were infected with the BoHV-1, vBoHV-1-∆UL21, or vBoHV-1-∆UL21R viruses at a MOI of 3. At 18 hpi, the number of virus particles in each well was calculated for 40 infected cells for each individual sample. *** indicates a statistically significant difference (p < 0.001). Bars indicate the mean values for each sample resulting from three independent experiments and error bars indicate the standard deviations. (A) Nucleocapsids in the nuclei. (B) Intracellular mature virions in the cytoplasm. (C) Mature virions on the cell surface.