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. 2017 Oct 10;8(55):94554–94568. doi: 10.18632/oncotarget.21791

Figure 6. MiR-139-5p inactivated the Wnt/β-catenin signaling pathway through directly targeting Wnt1.

Figure 6

(A) Bioinformatics analysis showed the predicted binding sites between miR-139-5p and Wnt1. (B) Luciferase reporter assay demonstrated that miR-139-5p overexpression significantly decreased the luciferase activity of Wnt1-WT in HEK293T cells. (C) MiR-139-5p overexpression decreased the mRNA expression of Wnt1 in T24 and 5637 cells by qRT-PCR assay. (D) The protein expression levels of tumor-related genes (Wnt1, β-catenin, and cyclin-D1) in the Wnt/β-catenin signaling pathway were suppressed in T24 and 5637 cells by miR-139-5p overexpression by western blot assay. Data are shown as mean ± SD. *P < 0.05, **P < 0.01.