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. 2017 Oct 17;8(55):94650–94665. doi: 10.18632/oncotarget.21841

Figure 5. MMP9 overexpression is associated with decreased paracellular permeability.

Figure 5

In vitro model of stably transfected human intestinal epithelial cells CaCo2BBE overexpressing MMP9 were used to assess paracellular permeability at 0.5 hour, 1 hour, 2 hours and 4 hours by using 4kD and 40kD FITC dextran molecule. (A) Flux of 4kD FITC ng/ml/min. (B) Flux of 40kD FITC ng/ml/min. Each bar represents mean ± S.E., *p< 0.05, **p< 0.005, ***p< 0.0005. The organization of TJs was assessed by performing WBs (30μg/lane) with whole cell lysates probed with (C i-iii) Claudin-2, Claudin-4 and Occludin respectively. The loading control for each blot was GAPDH. Each blot was a representation of three individual experiments. Densitometry evaluations of the WB is represented by the adjacent bar graph and each bar represents mean ± S.E., *p< 0.05. The integrity of TJs were studied by performing WBs (10μg/lane) for Triton X-100 soluble versus Triton X-100 insoluble fraction (D i-iii) Claudin-2, Occludin and Claudin-4 respectively.