Figure 1. YK-4-279 inhibits the viability and anchorage-independent growth of NB cells.
(A) Cell cytotoxicity of YK-4-279 of NB cells in the CCK-8 assay. Six human NB cell lines SK-N-AS, SH-SY5Y, CHLA-255, NB-19, NGP, and IMR-32 were treated with YK-4-279 at the concentrations of 0, 0.01 μM, 0.03 μM, 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, 30 μM, and 100 μM for 72 h, and then subjected to a CCK-8 assay. The absorbance of each well was measured at 450 nm and plotted for the cell viability curve. The data are represented as mean ± SD. IC50 values of YK-4-279 in NB cell lines are listed. (B) Colony formation of NB cells treated with YK-4-279. NB cells were seeded in 6-well plates at 5 ×103 per well, and then incubated with YK-4-279 at 0, 1 μM, or 3 μM for two weeks. The colonies were fixed, stained with crystal violet, and photographed. (C) Anchorage-independent growth was assessed by soft agar assay. NB cells were incubated with YK-4-279 at 0, 1 μM, or 3 μM in soft agar for three weeks, followed by staining with crystal violet and photographed. (D) The colonies were counted and plotted. Data were represented as mean ± SD. * P<0.05, ** P<0.01, *** P<0.001, by ANOVA and Dunnett’s multiple comparison post-test.