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. 2017 Nov 20;6:e29303. doi: 10.7554/eLife.29303

Figure 5. Plk1 self-priming of PBIP1 is influenced by Cyclin A/Cdk1 and MYPT1.

Figure 5.

(A) Western blots using whole cell lysates from U2OS before (CT) and after si-RNA-mediated Cyclin A-knockdown (CycA KD) of MYPT1 knockdown (MYPT1 KD) for the indicated protein targets. (B) Quantification of Western blots for knockdown efficiency and pPBIP1-Thr78 in the presence or absence of Cyclin A or MYPT1 (p<0.0001, Chi square contingency analysis). (C) U2OS before (Control) and after si-RNA-mediated Cyclin A knockdown (Cyclin A KD) or MYPT1-knockdown (MYPT1 KD) in prometaphase cells stained for DNA, centromere-specific human antisera (ACA), tubulin, and pPBIP1-Thr78 as indicated. Scale bar, 5 µm. (D) Quantification of the intensity of kinetochore pPBIP1-Thr78 staining in U2OS cells before (CT) and after si-RNA-mediated Cyclin A knockdown (CycA KD) or MYPT1-knockdown (MYPT1 KD) in prometaphase cells (n ≥ 100 kinetochores/condition. CT vs CycA KD p=0.01, CT vs MYPT1 KD p=0.001; unpaired, two-tailed t-test).