Antibodies
Mouse monoclonal anti-Actin (WB at 1:4000)	Seven hills bioreagents	Cat #LMAB-C4
Human anti-ACA (anti-centromere antibody; IF at 1:1000)	Gift, Geisel School of Medicine	n/a
Rabbit polyclonal anti-Aurora B (IF/WB 1:1000)	Novus Biologicals	Cat# NB100-294
Mouse monoclonal anti-Cyclin B (WB 1:1000)	Santa Cruz	Cat# SC-245
Rabbit polyclonal anti-Cyclin A (WB 1:1000)	Santa Cruz	Cat# SC-751
Rabbit polyclonal anti-MYPT1 (IF/WB 1:1000);	Santa Cruz	Cat# SC-25618
Mouse anti-MYPT1 pSer473 (IF/WB 1:1000)	Gift, Fumio Matsumura; Yamashiro et al. (2008)	n/a
Rabbit polyclonal anti-Nek2 (WB 1:1000);	Santa Cruz	Cat# SC-33167
Rabbit monoclonal anti-Chk2 pT68 (WB 1:1000);	Cell Signaling	Cat# C13C1
Rabbit polyclonal anti-MLF1 Interacting Proten/PBIP1 pThr78 (IF/WB 1:500);	Abcam	Cat# Ab34911
Rabbit polyclonal anti-Plk1 (IF/WB 1:1000);	Bethyl Laboratories	Cat# A300-251A
Mouse monoclonal anti-Plk1 pThr210 (IF/WB 1:500);	Abcam	Cat# Ab39068
Mouse monoclonal anti-Securin (WB 1:1000)	Abcam	Cat# AB3305
Mouse monoclonal anti- α-Tubulin (DM1α) (IF at 1:3000, WB at 1:4000).	Sigma Aldrich	Cat# T6199
Mouse monoclonal anti-Hec1 (IF at 1:500)	Novus	Cat# NB100-338
Secondary antibodies used for IF were goat anti-mouse; goat anti-human; goat anti-rabbit; donkey anti-rabbit; donkey anti-mouse; 488, 568, 647 (IF at 1:1000)	Alexa Fluor Life Technologies	Cat# A21445; A21202; A11008; A11013; A31573; A11014; A11032
Secondary antibodies used for WB were goat anti-rabbit IgG (H + L)-HRP conjugate and goat anti-mouse IgG (H + L)-HRP conjugate (WB at 1:1000).	Bio-Rad	Cat# 170–6515, 170–6516