Figure 1.

Tspan9 is not detectable in platelets and lung from Tspan9-deficient mice. Platelets from wild-type (WT), heterozygote (Het) and Tspan9-deficient (KO) mice were lysed in 1% Triton X-100 lysis buffer, the proteins separated by SDS-PAGE and western blotted for Tspan9 (upper panel) and α-tubulin to confirm equal protein loading (lower panel), using the Odyssey Infrared Imaging System. Mouse lung samples were homogenised in 1% Triton X-100 lysis buffer and western blotted as described for the platelets.