FIGURE 2:

SORB-1::GFP localizes to muscle dense bodies and other muscle attachment sites. (A) SORB-1 localizes to dense bodies and adhesion plaques between myocytes (arrowhead). Image was created from a Z-projection of 10 focal planes. (B) Zoom of boxed region in A. Fluorescent signal localizes along the depth of the dense body structures (arrowhead). (C) Composite of fluorescence and DIC imaging, identifying SORB-1::GFP in muscle arm attachments at the nerve ring (arrowheads). (D) Composite of fluorescence and DIC imaging, lateral view. SORB-1::GFP signal localizes to intestinal attachment sites of the stomatointestinal (si) and anal depressor (ad) muscles. (E) Hermaphrodite, ventral view. Signal localizes to attachments of the vulval muscles (v) and is diffusely punctate in the underlying uterus (ut). (F) Male, lateral view. SORB-1 localizes to attachments of the diagonal (arrowhead) and spicule-associated muscles (arrow) of the male tail. Scale bars = 10 μm.