FIGURE 6:

sorb-1 loss of function results in disorganization of thick filaments, M-lines, and, to some extent, thin filaments. Wild-type or sorb-1(gk769190) young adults were immunostained with antibodies to MYO-3 (thick filaments), UNC-89 (M-lines), UNC-95 (base of dense bodies and M-lines), DEB-1 (base of dense bodies), and ATN-1 (major and deeper portion of dense bodies) and reacted with fluorescent phalloidin (thin filaments). The sorb-1 mutant shows disorganization of thin filaments in most body wall muscle cells. The sorb-1 mutant shows disorganization of thick filaments and M-lines. Despite SORB-1 localizing to dense bodies, other dense body components (UNC-95, DEB-1, and ATN-1) show no obvious disorganization. Scale bar = 20 μm.