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. 2017 Dec 1;28(25):3634–3646. doi: 10.1091/mbc.E17-06-0399

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© 2017 Landino et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Overexpression of GFP-INCENP rescues cleavage furrow ingression in MKlp2-depleted cells. (A) Top, single z-plane micrographs taken from time-lapse movies of cells transiently expressing mCherry-Utrophin after treatment with a control or MKlp2-targeting siRNA. Time is indicated in minutes:seconds. Dashed lines were used to generate kymographs. Scale bar, 10 μm. Bottom, kymographs of mCherry-Utrophin fluorescence across the division plane. Yellow arrows represent cleavage furrow regression. Scale bars, 2.5 min (y-axis) and 10 μm (x-axis). (B) Top, single z-plane micrographs taken from time-lapse movies of cells transiently expressing GFP-INCENP after treatment with a control or MKlp2-targeting siRNA. Time is indicated in minutes:seconds. Dashed lines were used to generate kymographs. Scale bar, 10 μm. Bottom, kymographs of GFP-INCENP fluorescence across the division plane. Scale bars, 2.5 min (y-axis) and 10 μm (x-axis). (C) Top, single z-plane micrographs taken from time-lapse movies of cells transiently expressing GFP-INCENP CR after treatment with a control or MKlp2-targeting siRNA. Time is indicated in minutes:seconds. Dashed lines were used to generate kymographs. Scale bar, 10 μm. Bottom, kymographs of GFP-INCENP CR fluorescence across the division plane. Scale bars, 2.5 min (y-axis) and 10 μm (x-axis). (D) Quantification of the percentage of cells from A that successfully completed furrow ingression. n = 12 (control) and 15 (MKlp2 siRNA) cells, *p < 0.05. (E) Quantification of total percentage of cells from B that successfully completed furrow ingression. n = 14 cells for each condition, n = 8 (control) and 12 (MKlp2 siRNA) cells. (F) Quantification of total percentage of cells from C that successfully completed furrow ingression. n = 14 cells for each condition, *p < 0.05.