TABLE 1:
Yeast strains used.
| Strain | Genotype | Origin/Reference |
|---|---|---|
| CRM67 | W303, mat a, (leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15) | Lab collection |
| CRM2268 | W303, mat a, chs5Δ::natMx4 | Lab collection |
| CRM2688 | W303, mat a, trk1∆::LEU2 trk2∆::HIS3 | Madrid et al. (1998) |
| CRM2689 | W303, mat a, ena1-4∆::HIS3 | Yenush et al. (2002) |
| CRM1590 | W303, mat a, chs3Δ::natMx4 | Lab collection |
| CRM3056 | W303, mat a, rim101∆::kanMx4 | This study |
| CRM2888 | W303, mat a, NHA1-GFP::hphNT1 | This study |
| CRM2896 | W303, mat a, chs5Δ::natMx4 NHA1-GFP::hphNT1 | This study |
| CRM1278 | W303, mat a, chs3∆::URA3 chs5Δ::natMx4 | Lab collection |
| CRM3119 | W303, mat a, HXT7-GFP::hphNT1 | This study |
| CRM3121 | W303, mat a, chs5Δ::natMx4 HXT7-GFP::hphNT1 | This study |
| CRM3117 | W303, mat a, STL1-GFP::hphNT1 | This study |
| CRM3121 | W303, mat a, chs5Δ::natMx4 STL1-GFP::hphNT1 | This study |
| CRM3058 | W303, mat a, chs5∆::natMx4 rim101∆:: kanMx4 | This study |
| CRM3085 | W303, mat a, RIM101-3xHA::LEU2 | This study |
| CRM3086 | W303, mat a, chs5Δ::natMx4 RIM101-3xHA::LEU2 | This study |
| CRM3133 | W303, mat a, RIM21-2xGFP::kanMx4 | This study |
| CRM3153 | W303, mat a, RIM21-2xGFP::kanMx4 chs5Δ::natMx4 | This study |
| CRM3094 | W303, mat a, RIM13-GFP::kanMx4 | This study |
| CRM3096 | W303, mat a, chs5Δ::natMx4 RIM13-GFP::kanMx4 | This study |
| CRM3098 | W303, mat a, RIM20-GFP::kanMx4 | This study |
| CRM3100 | W303, mat a, chs5Δ::natMx4 RIM20-GFP::kanMx4 | This study |
| CRM3155 | W303, mat a, aps1∆::kanMx4 | This study |
| CRM3157 | W303, mat a, chs5Δ::natMx4 aps1∆::kanMx4 | This study |
| CRM2406 | W303, mat a, PIN2-GFP::hphNT1 | This study |
| CRM2507 | W303, mat a, chs5∆::natMx4 PIN2-GFP::hphNT1 | This study |
| CRM3198 | W303, mat a, RIM101-3xHA::LEU2 aps1∆::hphNT1 | This study |
| CRM3191 | W303, mat a, chs5∆::natMx4 RIM101-3xHA::LEU2 aps1∆::hphNT1 | This study |
| YAS2157 | YPH499, QDR2-GFP::TRP1 | This study |
| YAS2158 | YPH499, QDR2-GFP::TRP1 chs5∆::LEU2 | This study |
| YAS2202 | YPH499, ENA1-GFP::TRP1 | This study |
| YAS2241 | YPH499, ENA1-GFP::TRP1 chs5∆::LEU2 | This study |
| CRM3159 | YPH499, ENA1-GFP::TRP1 chs5∆::natMx4 | This study |
| YAS563-16a | YPH499, bch1∆::HIS5 (S. pombe) bch2∆::KAN (Tn 903) bud7∆::LEU2 (K. lactis) chs6∆::URA3 (K. lactis) | Trautwein et al. (2006) |
| YAS563-5a | YPH499, bch1∆::HIS5 (S. pombe) | Trautwein et al. (2006) |
| YAS1974 | YPH499, ppz1∆::HIS5 (S. pombe) | This study |
| YAS1975 | YPH499, ppz1∆::HIS5 (S. pombe) chs5∆::LEU2 | This study |
| YPH499 | Mat a, ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 | Spang lab |
| YAS431 | YPH499, chs5∆::LEU2 | Spang lab |
| CRM3273 | W303, mat a, RIM21-2xGFP::kanMx4 SEC7-mRuby2::URA3 | This study |
| CRM3274 | W303, mat a, RIM21-2xGFP::kanMx4 SEC7-mRuby2::URA3 chs5∆::natMX4 | This study |
| FCM603 | W303, mat a, RIM13::natMx4 | Lab collection |
| CRM3381 | W303, mat a, vps27∆::kanMx4 chs5∆::natMX4 | This study |
| CRM3382 | W303, mat a, vps27∆::kanMx4 | This study |