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. 2017 Jul 27;8(56):95135–95151. doi: 10.18632/oncotarget.19619

Figure 4. Chemerin induces intracellular calcium mobilization and stimulates MAPK and Akt signaling in neuroblastoma cells.

Figure 4

Intracellular calcium mobilization was measured in SK-N-SH cells with confocal laser scanning microscopy following the stimulation with 10nM chemerin without (A) and with (B) the prior addition of the calcium chelator EDTA. The arrow indicates the time point when chemerin was added. (C) Chemerin stimulates the phosphorylation of Akt, ERK1/2 and MEK1/2 in SK-N-AS cells in a dose-dependent manner. The cells were serum-starved for 24h prior to stimulation and samples were taken 5, 10, 20 and 30min after stimulation. Densitometric analysis of the protein bands was performed and the ratios between p-ERK1/2 and total ERK1/2, p-Akt and total Akt as well as p-MEK1/2 and β-actin were calculated. The values are displayed relative to the control=1. The experiments were performed three times with similar results.