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. 2017 Jul 12;8(56):95206–95222. doi: 10.18632/oncotarget.19199

Figure 9. The combination of teriflunomide and PF477736 is cytotoxic in SUM159 triple negative breast cancer cell line.

Figure 9

(A) Representative flow cytometry analysis of cell cycle distribution in SUM159 cells collected 48 hours after the beginning of exposure to vehicle, 25 μM TFN, IC10 PF477736 (2.5 μM), or their combination at the same concentrations. (B) Flow cytometry analysis for apoptosis/ necrosis. Cells were exposed to vehicle, 5 mM hydroxyurea as a positive control, 25 μM TFN, IC10 PF477736 or their combination, collected and stained with annexin V/7-AAD. Quantitation was performed with FlowJo software. Results are expressed as mean values ± SD of three independent experiments. *p < 0.05 and **p < 0.01 as determined by two-tailed unpaired t-test. (C) Western blotting analysis of caspase 3 cleavage in cell lysates prepared 72 hours after the beginning of the exposure to either compound, their combination or 0.1 μM positive control camptothecin (CPT).