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. 2017 Aug 14;8(56):95223–95246. doi: 10.18632/oncotarget.20255

Figure 12. Orai1, Stim1, TRPC1 and TRPC4 proteins are up-regulated in breast cancer-associated endothelial colony forming cells.

Figure 12

Western blot and densitometry depicting the significant elevation in Orai1 (A), Stim1 (B), TRPC1 (C), and TRPC4 (D) proteins in BC-ECFCs as compared to N-ECFCs. Blots for Orai1, Stim1, TRPC1 and TRPC4 representative of 4 different experiments are shown in the lower panel. Lanes were loaded with 20 μg of proteins. Major bands of the expected molecular weight were observed in both cell types. One additional band of 77 kDa was detected by anti-Stim1 in RCC-EPCs. When both Stim1 bands (77 and 100 kDa) were compared to the single band detected at 100 kDa in N-ECFCs, the expression of Stim1 protein became significantly higher in BC-ECFCs. Each bar in the upper panel represents the mean±SE of the densitometric analysis of four different experiments. The asterisk indicates p<0.01 (Student's t-test).